Composite
Part:BBa_K4229018:Design
Designed by: Nikita Edel Group: iGEM22_Freiburg (2022-09-28)
RBS-spyCatcherXiaI-RBS-TnaB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 893
Illegal AgeI site found at 783 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
e.Coli (MG1655 strain). This biobrick itself was synthetised. The use was taken out of a paper.
References
Yin H. et.al., "Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli", ACS OMEGA, vol.6, 20569−20576, 2021, https://doi.org/10.1021/acsomega.1c02679.